LBM-Broth Miller Size:1000g 21 pH (100 g/l) 5

21 pH (100 g/l) 5

Molecular cloning: a laboratory manual

Dilute 20ml TAE Buffer 50X with 980ml deionised water to make 1 litre of TAE Buffer

Each FM Drop-Out mixture contain all components except for one or two essential Amino acids

10 pH (100 g/l) 2

LBM-Broth Miller Size:1000g 21 pH (100 g/l) 5LBM Broth High Salt In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Final pH 7. 0 0. 2 at 25C Suspend 26 gram powdered medium in 1 litre distilled water Store dry at room temperature