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Improvement of a synthetic medium for Dictyostelium Discoideum
2 at 25°C Suspend 47
SD Broth / 2% Glucose w/o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose
Yeast extract 10 Peptone 20 Galactose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water
Weight 137
Pre Sporulation Medium Size:250g Improvement of a synthetic mediumPre Sporulation Medium is used for strains that do not sporulate well when grown on sporulation medium directly. Cells are grown 1 2 days on pre sporalation medium before transferring them to sporulation medium. Formula g l Yeast extract 10 Glucose 100 Potassium Acetate 20 Agar 20 Suspend 150 gram powdered medium in 1 litre distilled water Store dry at room temperature.