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Final concentration is 40mM Tris Base
5x is a 5x concentrate and has to be diluted to a 1x concentration and subsequently supplemented with an appropriate carbon source
3-AT is a competitive inhibitor of the product of the HIS3 gene
G-418 DiSulphate Solution is a member of the large family of Aminoglycoside antibiotics and has a molecular structure almost like Gentamycin
Soluble in water
Tryptone Top Agar Size:1000g Final concentration is 40mM TrisFormula g l Tryptone 10 NaCl 8 Agar 7 Final pH 7. 0 0. 2 at 25C Suspend 25 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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