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16 pH (100 g/l) 7
CSM is also a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants
preventing peptide bond formation by peptidyl transferase
K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium
protect against moisture
YPAD Agar Size:250g 16 pH (100 g/l) 7Formula g l Yeast extract 10 Peptone 20 Glucose 20 Adenine Sulfate 0. 04 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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