Yeast Nitrogen Base without Amino acids and without para Amino Benzoic acid Size:1000g FM Amino acid drop-out mixture

FM Amino acid drop-out mixture is based on the Amino acids present in FM Minimal medium

Character Fine white powder Solubility Freely soluble in water Specific Optical Rotation 104 ~ 121 Water <6% Absorbance 570 nm <0

0 g/L MgSO4

05 Total 30

CSM is also a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants

Yeast Nitrogen Base without Amino acids and without para Amino Benzoic acid Size:1000g FM Amino acid drop-out mixturePara Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base w o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para Amino Benzoic acid or to replace this vitamin. Suspend 6. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature